Abstract
The hemagglutination inhibition immunoassay and the staphylococcal clumping technic for determining fibrinolytic split products both utilize the microtiter system. The quality control of this system, using 131I-labeled albumin, is described. Three dropper pipettes were found to have errors, based on an expected volume of 25 µ l., of –7.6 to 14.4% (mean 3.5%). Eight transfer pipettes were found to contain volumes larger than the expected 25 µ l.; the errors ranged from 6.4 to 14.0% (mean 9.9%). The proper use of the microtiter system and the accuracy of doubling dilutions are discussed. The technic has inherent pitfalls but, if performed properly, gives reproducible results.
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