Pit membrane chemistry influences the magnitude of ion-mediated enhancement of xylem hydraulic conductance in four Lauraceae species

Abstract

The ion-mediated enhancement of xylem hydraulic conductivity in angiosperms is thought to be controlled by the pectin chemistry of intervessel pit membranes. However, there is little or no direct evidence on the ultrastructure and chemical nature of pit membranes in species that show an 'ionic effect'. The potential link between the magnitude of the ionic effect and pectin composition in intervessel pit membranes of four Lauraceae species (Laurus nobilis, Lindera megaphylla, Litsea sericea and Umbellularia californica) that show rather similar vessel and pit dimensions was studied using transmission electron microscopy (TEM). The TEM observations confirmed the presence of a pectic matrix associated with intervessel pit membranes, indicating that the relative abundance of acidic versus methylesterified pectins was closely related to the ionic effect. The two species examined with a high ionic effect ~20%, i.e. Laurus nobilis and Umbellularia californica) showed relatively high levels of acidic pectins, whereas methylesterified pectins were abundant in Lindera megaphylla and Litsea sericea, which showed a low ionic effect (~10%). Variation in the ionic effect is strongly associated with the chemical nature of pit membrane pectins in the species studied. Our findings support the current interpretation of the ionic effect due to dynamic swelling and shrinking behaviour of pit membrane pectins.