Abstract
Pea ( Pisum sativum) tissues, on treatment with aqueous CuCl 2 synthesize the 6a-hydroxypterocarpan phytoalexin (+) - (6aR, 11aR) - pisatin. By supplying (−) - (6aR, 11aR) - maackiain during this induction process, sigruficant quantities of ( − ) - (6aS, 11aS) - pisatin are produced, immature pods being most effective. Pisatin levels are considerably reduced when compared with the normal induction process, but may contain as much as 92% (−)-pisatin. This confirms that the 6a-hydroxylation of maackiain during the biosynthesis of pisatin must proceed with retention of configuration at C-6a.
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