PiRNA-31115 Promotes Cell Proliferation and Invasion via PI3K/AKT Pathway in Clear Cell Renal Carcinoma.

Xinghua Du,Baoli Heng,Caiyong Lai,Xuexia Xie,Haomin Li,Guoliang Li,Liping Shi,Fan Wu,Xue Gao

doi:10.1155/2021/6915329

Abstract

PIWI-interacting RNAs (piRNAs) are small noncoding RNAs that play important roles in germline development and carcinogenesis. In this study, we used the deep sequencing of small RNA Transcriptome to explore the piRNA expression in six clear cell renal carcinoma (ccRCC) tissues and matched adjacent normal tissues and found that six piRNAs were upregulated and sixteen were downregulated in ccRCC tissues. Among them, piRNA-31115 (NCBI accession number: DQ571003) was the most upregulated piRNA in ccRCC tissues compared with matched adjacent normal tissues. Quantitative real-time PCR (qRT-PCR) was used to confirm piR-31115 expression in other ccRCC tissues (n = 40) and ccRCC cell lines. Besides, function analysis demonstrated that silencing of piR-31115 inhibited ccRCC cell proliferation, motility, and invasiveness. Mechanistic investigations showed that piRNA-31115 may activate epithelial-mesenchymal transition (EMT) via the PI3K/AKT signaling pathway. Hence, piR-31115 may represent an oncogene in the development of ccRCC.

Highlights

Renal cell carcinoma (RCC) is one of the most common malignant tumors of the urinary system, representing approximately 2% to 3% of all adult malignancies [1, 2]
19 PIWI-interacting RNAs (piRNAs) were upregulated and 41 piRNAs were downregulated in Clear cell renal cell carcinoma (ccRCC) tissues; all the piRNAs are listed in the supplementary materials
PiRNA-31115 was the most upregulated piRNA in ccRCC tissues compared with matched adjacent normal tissues (Figure 1(a))

Summary

Introduction

Renal cell carcinoma (RCC) is one of the most common malignant tumors of the urinary system, representing approximately 2% to 3% of all adult malignancies [1, 2]. Clear cell renal cell carcinoma (ccRCC) is the most common type of RCC, which accounts for 60% to 85% of all RCC cases [3] Current imaging methods, such as computed tomography (CT), have been able to detect more and more small renal masses; ccRCC is insidious, and about 30% of patients will develop local invasion and distant metastatic lesions even after they are found [4, 5]. These ccRCC patients have already lost their chances of radical surgical resection and exhibit a low fiveyear survival rate. PiRNAs play an important role in the regulation of gene expression via TE silencing and epigenetic modifications

Methods

Results

Discussion

Conclusion