Piriformospora indica, a cultivable root endophyte with multiple biotechnological applications

Abstract

Piriformospora indica is a wide-host root-colonizing endophytic fungus which allows the plants to grow under extreme physical and nutrient stress. The fungus can be cultivated on complex and minimal substrates. It belongs to the Sebacinales in Basidiomycota. P. indica has a vast geographical distribution and is reported from Asia, South America and Australia. The fungus is interesting for basic research as well as biotechnological applications because: (i) it functions as a plant promoter and biofertilizer in nutrient-deficient soils, (ii) as a bioprotector against biotic and abiotic stresses including root and leaf fungus pathogens and insect invaders, (iii) as a bioregulator for plant growth development, early flowering, enhanced seed production, and stimulation of active ingredients in medicinal plants (iv) as well as a bio-agent for the hardening of tissue-culture-raised plants. Positive interaction are established for many plants of economic importance in arboriculture, agro-forestry, flori-horticulture including Orchids, and those utilized for energy production and paper industry. P. indica also interacts with members of bryophyte, Aneura pinguis, pteridophyte, Pteris ensiormis, Gymnosperms (Pinus halepensis) and a large number of angiosperms (145 tested till date) including the model plant Arabidopsis thaliana and other members of the mustard family. Similar to arbuscular mycorrhizal fungi, P. indica stimulates nutrient uptake in the roots and solubilizes insoluble phosphatic and sulphur components in the soil. The interaction of P. indica with the model plants Arabidopsis thaliana and barley (Hordeum vulgare L.) is used to understand the molecular basis of this beneficial plant/microbe interaction. We describe the current knowledge about the molecular basis of the interaction of plants with P. indica. An attempt is made to compare it with pathogenic and mycorrhizal plant/microbe interactions and also propose possible biotechnological applications.