Abstract
The paper focuses on the pioneer evaluation of the possible side effects of a novel preparation against phyllophagous insects, DNA insecticides, on some biochemical parameters (concentration of glucose and activity of alkaline phosphatase) of wheat sprouts (Triticum aestivum L.). Our results show that the application of DNA insecticide based on single-stranded DNA fragment from RING domain of IAP-3 gene of gypsy moth multicapsid nuclear polyhedrosis virus results in significant difference in the decreased level of glucose and activity of alkaline phosphatase in wheat sprouts in comparison with the control after treatment on the second day of the experiment. However, the effect becomes significantly unnoticeable after one week when compared with the control. No significant difference in the dried biomass was found between wheat sprouts from the control and the experimental groups after the treatment on the seventh day and after three weeks on the Hoagland's substrate. Obtained results generally show that agricultural plants are able to overcome the misbalance in the concentration of glucose and activity of alkaline phosphatase caused by the DNA insecticides and emphasize good potential in harmlessness of DNA insecticides that could be used to control phyllophagous insects without substantial side effects on the crop.
Highlights
Increasing the efficiency of agricultural and forest defense against pest insects is vitally and economically very important
Biopreparations based on viral anti-apoptotic gene fragments, termed DNA insecticides, could be a safe, inexpensive and fast alternative to baculoviral preparations means of struggle against phyllophagous insects (Oberemok 2008; Oberemok 2009; Oberemok 2011; Simchuk et al 2012; Oberemok et al 2013)
Relationships between baculoviruses and their insect hosts are subject to coevolution, this should lead to long-term evolutionary effects such as the specialization of these pathogens for their hosts (Herniou et al 2004), and the ability to affect their biochemical reactions through expression of homologous anti-apoptotic genes
Summary
Increasing the efficiency of agricultural and forest defense against pest insects is vitally and economically very important. DNA insecticides have good potential to be harmless to the members of an ecosystem since deoxyribonucleic acid is the molecule found in every cell and with distinct sequences of nitrogenous bases we target natural mechanisms involved in expression of distinct genes in distinct organisms In this regard, the following research paper is devoted to the pioneer investigation of the influence of DNA insecticides on some biochemical parameters and biomass accumulation of seedlings of wheat, as the most vulnerable stage of the plant (Lianes et al 2005), so as to evaluate the possible side effects of the DNA insecticides on the crop
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