Abstract
The analysis of altered glycosylation patterns may provide biomarkers for various types of cancer. The present study developed a Pinellia pedatisecta agglutinin (PPA)-based lectin blot analysis technique, which was used to analyze the glycosylation patterns in various types of cancer cells. Results showed that a typical band located between 47 and 85 kDa was obtained in the HL60 leukemia cells, whereas three typical bands located between 20 and 47 kDa were observed in the Kasumi-1 leukemia cells. For the PLC, BEL-7404, Huh7 and H1299 solid tumor cell lines, different band patterns were detected, with bands typically located between 55 and 100 kDa. The findings of the present study show that PPA-based lectin blot analysis is capable of distinguishing between glycosylation patterns in leukemia and solid tumor cell lines. The glycofiles detected using PPA-based lectin blot analysis may provide a ‘glycosylation fingerprint’ for a variety of cancer cells, which may be valuable for cancer prognosis and diagnosis.
Highlights
Altered glycosylation has been reported in various types of cancer and may have a role in cancer metastasis and progression [1,2,3]
The solid tumor cell lines, including the PLC, BEL‐7404 and Huh7 liver cancer cell lines and the H1299 lung cancer cell line, were further analyzed using Pinellia pedatisecta agglutinin (PPA)‐based lectin blot analysis. These four cell lines exhibited different glycosylation patterns and the bands were primarily between 55 and 100 kDa. These findings indicate that PPA‐based lectin blot analysis could be used to distinguish between glycosylation patterns in various cancer cell types, including types of blood cancer and solid tumors
SCAR‐PPAb, soluble coxsackie‐adenovirus receptor‐Pinellia pedatisecta agglutinin domain b; MW, molecular weight. Due to their oligosaccharide specificity, lectins have been used in a variety of biological techniques, including lectin array, lectin blot analysis and lectin‐based chromatography [17]
Summary
Altered glycosylation has been reported in various types of cancer and may have a role in cancer metastasis and progression [1,2,3]. Lectins are carbohydrate‐binding proteins that contain at least one non‐catalytic domain that binds reversibly with mono‐ or oligosaccharides with high specificity [4]. Lectins may be useful tools for analyzing glycofiles and may be used as biomarkers for a variety of types of cancer, including aggressive breast [5,6], ovarian [7], pancreatic [8], prostate [9] and liver [10] cancer. The monocot mannose‐binding lectin, Pinellia pedatisecta agglutinin (PPA), accumulates in These studies indicate that PPA may be further developed to analyze glycosylation profiles in different types of cancer
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