Abstract
Pineal hydroxyindole-O-methyltransferase was measured in White-Leghorn cockerels exposed to a diurnal lighting cycle. The effect of varying substrate concentrations was also determined, and optimal concentrations for both N-acetylserotonin and 5-hydroxytryptamine were observed. The apparent Km for NAS was approximately 7000 times lower than that for 5HT. When HIOMT activity is measured at different times of day, a diurnal rhythm is detected with maximum activity during the dark phase of the photoperiod.
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