Abstract
Pilot testing of a microbiological method for disposal of spent hydrocarbon oils (HO) by a previously developed consortium of hydrocarbon oxidizing microorganisms (HOM): Aquamicrobium lusatiense strain 854/1 (AM884147); Pseudomonas aeruginosa strain HNYM10 (JN 999888), Paracoccus aminophilus strain ATCC 7061 (NR_043242), Gordonia hydrophobica strain DSM44015 (NR_926254), Bacillus pumilus strain ATCC 7061 (NR_043242). When cultivated under flow conditions in liquid mineral medium with spent oil, their population increased from 1,5–2·104 CFU/ml to 5–6·109 CFU/ml, which was accompanied by a significant increase in their enzymatic activity. The effectiveness of biodegradation of oil at its initial concentration of 25 and 50 wt. % was 93–94 % during 60 days. All hydrocarbons in the composition of the oil to some extent underwent microbiological oxidation, and, depending on the structure, the degree of their disposal was 82–100 %.
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