Abstract

Micropipettes, or piston-pipettes, are used in analytical laboratories for medical applications when it is necessary to deliver liquid in the range of the microliter. In order to determine the exact delivered volume of each micropipette it is necessary to calibrate them using the appropriate methods described in ISO 8655[1]. One of the methods is the photometric method which uses a high-resolution photometer and colorimetric solutions to determine the volume delivered by a micropipette. Considering that there is still some lack of knowledge about the performance of this method by the National Metrology Laboratories, it was organized, by IPQ and Artel, a pilot study to verify the applicability of this method in each laboratory.

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