Pilot Study of Biomarkers of Oxidative Stress and Chlamydia trachomatis in Female Patients with Spontaneous Abortions

Abstract

The aims of the present study were i. asses the biomarkers of oxi- dative stress including superoxide anion radical, Nitrite measurement and reduced glutathione in female patients with spontaneous abortions Asses the prevalence of C. trachomatis antibodies IgA and IgG in those patients, iii. Correlate the levels of the oxidative biomarkers to the presence of C. trachomatis antibodies. The study included one hundred female patients with 2 or more spon- taneous abortions in addition to one hundred healthy control females with normal gravidity and parity history. Blood samples were obtained from each subject and subjected to laboratory determination of super- oxide anion radical, Nitrite measurement and reduced glutathione by biochemical methods. Determination of specific immunoglobulin A and G (IgA, IgG) was determined by enzyme linked immunosorbent assay (ELISA). The prevalence of IgA and IgG for C. trachomatis in patients was 4% and 8% respectively and, in the control, subjects the prevalence of IgA and IgG were 1% and 2% respectively. The concentration of oxidative stress products was significantly higher in the patients group compared to the control group (P=0.0001). The concentrations of nitrite and reduced glutathione were 27.9± 4.7, 31.4± 1.9 nmol/ml respectively in patients and the concentrations of nitrite and reduced glutathione were 18.9± 2.1 and 27.9± 3.9 respectively in the control group. The concentration of SOD was significantly reduced in the patients group compared to the control group (P=0.0001). The concentration of SOD was 55.1 6.9 in the patients and in the control the concentration was 64.5± 7.7 nmol/ml In the study of oxidative stress markers in the patients with positive serology for C. trachomatis, there was significant increase in nitrite concentrations compared to patients negative for C. trachomatis sero- logical markers (P=0.005). While reduced glutathione concentration had insignificant increase in patients positive for C. trachomatis with reduced SOD compared to patients with negative serology to C. tra- chomatis, P=0.6, P=0.07, respectively. The present study highlights that there was significant increase in oxi- dant stress biomarkers nitrite and reduced glutathione with significant reduction in superoxide dismutase in patients with repeated abortions. The prevalence of IgG to C. trachomatis was significantly prevalent in patients with recurrent abortion compared to control subjects. The nitrite was significantly correlated with positive serology to C. tracho- matis.