Abstract
Dormant buds of 64 apple accessions from the National Germplasm Repository (NGR), Geneva, NY were cryopreserved at the National Seed Storage Laboratory (NSSL), Fort Collins, Co. Initial tests after 1 mon, 1, 2, and 3 years of LN2 storage showed no decline in viability. Storage of 16 cultivars (1988/89 and 1989/90 dormant seasons) with a broad range of cold-hardiness characteristics has shown approx 45% viability by patch budding. Storage from dormant seasons of 1990/91 and 1991/92 included 48 cultivars selected for excellent cold-hardiness characteristics. With approx 85% initial viability of these cultivars, a more sensitive statistical analysis can be performed over years. Overall viability over storage duration and sampling years showed 32 had more than 80%, 55 had more than 50% and only 4 had less than 30%.
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