PIII-2 Diagnosing human papillomavirus and human papillomavirus type 16 by real-time PCR in patient undergone to colposcopy and significance of the diagnosis

Abstract

SUMMARY Objective: It is aimed to determine presence of HPV and HPV 16 by Real-Time PCR in cervical smears obtained from patients during colposcopic examination who had referred to outpatient clinic of Gynecology and Obstetrics Department due to various complaints and to examine interrelation between positive test results and clinical data. Material and methods: Sixty patients were included in the study who were referred to outpatient clinic due to varied complaints and who had been decided to undergo colposcopic examination. DNA was obtained from each smear sample by phenol-chloroform-isoamylalcohol method. L1 region was amplified in amplification process using MY09/MY11 primers. Products for Nested Real time PCR were studied in Light Cycler by GP5+/GP6+ primers and Cyanine-5 labeled HPV 16 DNA specific probe. Real time PCR products underwent melting curve analysis by LightCycler software version 3.5.3. HPV DNA positivity and HPV 16 positivity were determined at 78- 82°C and 68°C, respectively. Results: No statistically significant difference could be detected between HPV positivity, HPV 16 and types other than HPV 16 in control group and patients with positive test result as a consequence of colposcopic examination. Again, no statistically significant difference could be detected between HPV positivity and status of parity, PAP test result, marital status and age of patient. Conclusion: No statistically significant difference could be detected between HPV positivity, HPV 16 and types other than HPV 16 in control group and patients with positive test result as a consequence of colposcopic examination. Again, no statistically difference could be detected between HPV positivity and result of PAP smear test, marital status, age of patient and smoking but statistically significant difference could be detected between types other than HPV 16 and status of parity (respectively; ρ2=0.821, p=0.365; ρ2=0.752, p=0.564; ρ2=0.364, p=0.834; ρ2= 6.835, p=0.033).