Abstract
1. 1. Monomeric pigeon liver purine nucleoside phosphorylase did not exhibit the phenomenon of substrate activation at high substrate concentrations. 2. 2. Initial velocity studies and product inhibition patterns for the synthesis of inosine were consistent with a Random Bi, Bi mechanism. 3. 3. One mole of native purine nucleoside phosphorylase binds with 2 mol of 5,5′-dithiobis-(2-nitro-benzoic acid) and there is an increase in number upon denaturation with sodium dodecyl sulfate. 4. 4. Hypoxanthine, inosine, orthophosphate and ribose-1-phosphate partially protect the sulfhydryl groups from reaction with 5,5′-dithiobis-(2-nitrobenzoic acid). 5. 5. p-Chloromercuribenzoate and 5,5′-dithiobis-(2-nitrobenzoic acid) inhibit the activity of the pigeon liver enzyme according to a second order reaction kinetics.
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