Piégeage des radicaux libres et activité cytotoxique des graines extraites du Podophyllum hexandrum

Abstract

In the present study, in vitro antioxidant and cytotoxic activities of different extracts of Podophyllum hexandrum seed were investigated. Different polarities crude extracts were prepared through Soxhlet extractor and were used for the determination of antioxidant potential by 2,2′-diphenyl-1-picrylhydrazy (DPPH), reducing power, microsomal lipid peroxidation, and hydroxyl radical scavenging activity. Cytotoxic activity was carried out by the Sulphorhodamine B (SRB) method. The highest phenolic content of 465 ± 5.55 mgGallic Acid Equivalent(GAE) /g was observed in methanol extract followed by ethanol (359 ± 8.52) and aqueous extract (291 ±4.5 mgGAE/g). At a concentration of 700 μg/ml, DPPH radical scavenging activity was found to be highest in methanol extract (86 ±1.5%) followed by ethanol extract (78 ± 1.2), aqueous extract (71 ± 1.5), and ethyl acetate extract (64 ± 1.5%). At a concentration of 300 μg/ml, 76%, 65%, 65%, and 60% inhibition on microsomal lipid peroxidation was observed with methanol, ethanol, aqueous, and ethyl acetate extracts, respectively. Cytotoxicity of the extracts (100 μg/ml) was tested on three human cancer cell lines (MCF-7, HCT-116, and HeLa) using the SRB assay. These results clearly indicate that Podophyllum hexandrum seed extracts possess free radical savaging and cytotoxic activity and can be used against various oxidative stressrelated pathological conditions.