Piezoelectric properties of collagen-nanocrystalline hydroxyapatite composites

Abstract

In this paper we did a study of the physicochemical, dielectric and piezoelectric properties of anionic collagen and collagen-hydroxyapatite (HA) composites, considering the development of new biomaterials which have potential applications in support for cellular growth and in systems for bone regeneration. The piezoelectric strain tensor element d14, the elastic constant s55, and the dielectric permittivity ɛ11 were measured for the anionic collagen and collagen-HA films. For the collagen-HA composite film (Col-HACOM) the main peaks associated to the crystalline HA is present. For the nanocrystalline composite, nanometric HA powder (103 nm particle size) (HAN), obtained by mechanical milling were used. For the composite film (Col-HAN) the HA and CaH(PO4)2H2O phases were detected. One can see that the HA powder (HAN) present the main peaks associated to crystalline HA. The IR spectroscopy measurements on HA-COM and HAN powders, Col-HACOM and Col-HAN composite films and collagen film (Col) presents the main resonances associated to the modes of (PO4)3−, (CO3)2−. The IR spectra of Collagen Film (Col) shows the bands associated to amide I (C=O), amide II (N–H) and amide III (C–N) vibrational modes. The scanning electron photomicrography of the Col-HACOM and Col-HAN films, respectively, shows deposits of HA on the surface of collagen. It also shows that HACOM crystals has a dense feature, whereas the HAN crystals has soft porous surface. Energy-dispersive spectroscopy (EDS) analysis showed that the main elements of the hybrid sponge were carbon, oxygen, calcium, and phosphorus. The EDS of HACOM crystal, present in the Col-HACOM composite showed a molar ratio Ca/P = 1.71, whereas the Col-HAN composite the molar ratio of calcium and phosphorus (Ca/P = 2.14) and the amount of carbon were greater. The piezoelectric strain tensor element d14 obtained for the anionic collagen was around 0.102 pC/N. The collagen composite with nanocrystalline HA crystals (Col-HAN) present a better result (d14 = 0.040 pC/N) compared to the composite with the commercial ceramic (d14 = 0.012 pC/N). This is an indication that the nanometric particles of HA present little disturbance on the organization of the collagen fibers in the composite. In this situation the nanometric HA are the best candidates in future applications of these composites.