Abstract
Detection of mechanical stress is essential for diverse biological functions including touch, audition, and maintenance of vascular myogenic tone. PIEZO1, a mechano-sensing cation channel, is widely expressed in neuronal and non-neuronal cells and is expected to be involved in important biological functions. Here, we examined the possibility that PIEZO1 is involved in the regulation of synovial sarcoma cell-viability. Application of a PIEZO1 agonist Yoda1 effectively induced Ca2+ response and cation channel currents in PIEZO1-expressing HEK (HEK-Piezo1) cells and synovial sarcoma SW982 (SW982) cells. Mechanical stress, as well as Yoda1, induced the activity of an identical channel of conductance with 21.6 pS in HEK-Piezo1 cells. In contrast, Yoda1 up to 10 μM had no effects on membrane currents in HEK cells without transfecting PIEZO1. A knockdown of PIEZO1 with siRNA in SW982 cells abolished Yoda1-induced Ca2+ response and significantly reduced cell cell-viability. Because PIEZO1 is highly expressed in SW982 cells and its knockdown affects cell-viability, this gene is a potential target against synovial sarcoma.
Highlights
Detection of mechanical stress is essential for diverse biological functions including touch, audition, and maintenance of vascular myogenic tone
We examined the possibility that PIEZO1 is involved in the regulation of synovial sarcoma cell-viability
The Ca2+ response of HEK-Piezo1 cells to Yoda1 was significantly attenuated in standard HEPES-buffered bathing solution (SBS) without Ca2+ (Figure 1C), confirming that Yoda1 is an effective activator of human PIEZO1 as previously reported [6,20]
Summary
Detection of mechanical stress is essential for diverse biological functions including touch, audition, and maintenance of vascular myogenic tone. In the latter, shear stimuli into vascular endothelial cells can activate various endothelial ion channels and subsequently affect vascular functions. PIEZO2 is predominantly expressed in sensory tissues It is a mechano-sensor in Merkel cells and plays a key role in mediating the moderate touch sensation on the skin [13,14,15]. By employing PIEZO1 agonist Yoda and siRNA technology, we demonstrate that PIEZO1 is highly expressed in human synovial sarcoma SW982 cells and its knockdown affects the cell-viability
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
