Abstract
Piericidin A1, 3’-rhamnopiericidin A1, and a novel compound piericidin E, a new quorum-sensing (QS) inhibitor against Chromobacterium violaceum CV026, were isolated from the culture broth of Streptomyces sp. QS is well known as a microbial signaling system and controls certain types of gene expression resulting in bioluminescence, biofilm formation, swarming motility, antibiotic biosynthesis, and virulence factor production. C. violaceum CV026 is commonly used to determine qualitative and quantitative QS activity. The structures of piericidin derivatives were characterized, and their QS activities were determined.
Highlights
Quorum sensing (QS) is a process for cell-to-cell communication in bacteria using intermediary substances, which are excreted from bacterial cells into the environment
We screened metabolites in the culture broth of about 1000 strains of Actinomycetes isolated from soils for their capacity to inhibit the production of violacein in Chromobacterium violaceum CV026 [12,13] and found that the culture broth from 103 strains produced QS inhibitors (QSIs)
200 μL of LuriaBertani soft agar (LSA) containing C. violaceum CV026 and HHL were added, and the microtiter plate was cultured at 27 ̊C until the purple pigment in the control was well developed
Summary
Quorum sensing (QS) is a process for cell-to-cell communication in bacteria using intermediary substances, which are excreted from bacterial cells into the environment. When the concentration of such an excreted metabolite reaches its threshold level, certain types of gene expression resulting in bioluminescence [1], biofilm formation [2,3], swarming motility [4], antibiotic biosynthesis [5,6] virulence factor production [7,8], etc., are triggered by the metabolite (autoinducer). Disruption of the QS system in pathogenic Burkholderia cepacia and B. pseudomallei resulted in reduced pathogenicity in murine and hamster infection [9,10], and erythromycin inhibits biofilm formation of Pseudomonas aeruginosa below the minimum inhibitory concentration (MIC) [11]. We screened metabolites in the culture broth of about 1000 strains of Actinomycetes isolated from soils for their capacity to inhibit the production of violacein in Chromobacterium violaceum CV026 [12,13] and found. The structures of piericidin A1 and its derivatives isolated as QSIs were characterized, and the QS inhibitory activity and taxonomical properties of the strains that produced these QSIs were analyzed
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