Abstract
An experimental apparatus for measuring time-resolved circular dichroism (CD) with picosecond resolution is described. The time resolution of the device is determined by the laser pulse width, not the modulation frequency of an electro-optic or piezo-optic crystal as is the case for commercial CD spectrometers. The time-dependent CD signal can be monitored over the wavelength range from 280 to 850 nm. The data from single wavelength studies can be pieced together to generate time dependent spectra. The experimental approach is compared to previous time-resolved CD techniques which, for technical reasons, have been limited to, at most, nanosecond resolution. The picosecond apparatus is shown to be free of many of the polarization artifacts present in these previous devices. The utility of this technique is demonstrated by examining result on the time dependent near-UV CD of myoglobin following the photoelimination of coordinated CO from carbonmonoxymyoglobin.
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