Abstract
Swelling-induced Cl−currents were investigated in freshly prepared non-pigmented epithelial (NPE) and pigmented epithelial (PE) cells of the rabbit ciliary body using the whole-cell patch clamp technique. Exposure of both NPE and PE cells to hypotonic stress induced Cl−currents that exhibited outward rectification and were insensitive to Ca+2. We found that swelling-induced Cl−currents in PE cell are observed shortly after isolation. The swelling-induced Cl−current showed little or no inactivation at positive membrane voltages and was sensitive to 100 μM NPPB and 100 μM DIDS. Injection of cRNA encoded rabbit pIClninto Xenopus oocytes produced an outwardly rectifying Cl−current displaying features consistent with the swelling-induced Cl−current in epithelium. pIClnis ubiquitous in the ciliary epithelium. It participates in the equilibration of short term tonicity alterations, a phenomenon underlying mechanisms with larger and slower amplitudes for aqueous secretion by these cells.
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