Abstract
Abstract Pichia anomala is antagonistic against a range of spoilage molds in vitro as well as against Penicillium roqueforti in high-moisture wheat during malfunctioning airtight storage in laboratory experiments. The use of Pichia anomala to improve the postharvest control of Penicillium roqueforti during airtight storage of feed grain was evaluated in outdoor silos. Inoculated and control winter wheat (cultivar Kosack) in 160-kg portions were stored at a water activity of 0.93 for 12 months in silos that were opened twice a week. During the first 2 months, inoculated Pichia anomala increased to about 107 colony-forming units (CFU)/g, while naturally occurring Pichia anomala in the treatments without inoculated yeast increased from 104 to 106 CFU/g. During the same period, CO2 concentrations increased to almost 70% and stabilized at 50–60%. During the coldest period, O2 concentrations of
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