PI-31Genotype frequencies for ten polymorphisms of the renin-angiotensin-aldosterone system between healthy French-Canadians and other ethnic groups

Abstract

BACKGROUND/AIMS Differences exist in frequencies of certain genotypes within different populations. We analysed the polymorphism frequency of ten polymorphisms of the RAAS in our population and compared them to other ethnic groups. METHODS This is an observational study performed on 18 to 25 years old 200 healthy males of French-Canadian origin. The Gen Elute Blood Genomic DNA kit (Sigma PC# NA2020) was used to extract DNA. The studied polymorphisms were: ACE I/D, ATR1 A1166C, AGT M235T, AGT T174M, CYP11B2 T-344C, α-adducin G460W, beta-2 adrenergic receptor Q27E, bradykinin β2R +9/-9, eNOS T-786C and eNOS E298D. They were analysed by the standard PCR/enzymatic digestion/electrophoresis method. RESULTS All polymorphisms tested were in Hardy-Weinberg equilibrium. When compared to other ethnic groups, our population's genotype frequencies differed from the Asian and the African-American populations with respect to every polymorphism, except for the bradykinin β2R gene (data was unavailable) and differed from the African population with respect to the polymorphism of ACE, AGT235, AGT174 and CYP11B2. When compared with other Caucasian populations, we obtained different genotype frequencies for the ATR1, AGT235, beta-2 adrenergic receptor, eNOS-786 and eNOS298 genes. CONCLUSION Our study depicts the genetic profile of ten polymorphisms of the RAAS in French-Canadian healthy man. We noted differences in that profile when compared to Africans, African-Americans, Asians and other Caucasians. Clinical Pharmacology & Therapeutics (2005) 79, P15–P15; doi: 10.1016/j.clpt.2005.12.052