PI-2 Comparison of type-specific HPV detection in urine and vulvo- vaginal samples from adeolescent girls in Jos, Nigeria

Abstract

Objective: To compare self-collected vulvo-vaginal swab and first void urine for the detection of HPV among adolescent girls in Jos. Methods: We randomly recruited 205 girls, age 9–20 years from 10 public and private high schools in Jos, Nigeria. After informed consent and assent, each participant self-collected vulvo-vaginal samples and first-void urine which were tested using SPF10-DEIA/LiPA25. Data were analyzed using STATA14. Results: The mean (SD) age of participants was 14.9 (2.3) years. We detected anyHPV in 13.2% of vulvo-vaginal and 21.0% of urine samples. 7.8% and 11.2% of vulvo-vaginal swab and urine had hrHPV respectively. The commonest hrHPV types in vulvo-vaginal swab were 52 (3.9%), 51 (2.4%) and 18 (1.5%), and in urine were 52 (6.8%), HPV 18 (5.4%) and HPV 51 (2.9%). Approximately 10% and 14% of vulvo-vaginal samples and urine samples had single infections; and 3.4% and 7.3% had multiple infections respectively. Multiple hrHPV infection was detected in 2.9% of vulvo-vaginal swab and 5.9% of urine samples. Of the 27 vulvo-vaginal swabs and 43 urine samples positive for anyHPV, in only 10 (37.0%) instances were both vulvo-vaginal and urine samples positive for anyHPV. There was poor concordance between the urine and vulvo-vaginal samples for anyHPV (κ = 0.15, P = 0.03). Conclusions: The poor concordance suggests that first-void urinary HPV is not a suitable proxy for vulvo-vaginal HPV infection in this population.