Phytotoxicity, nematicidal activity and chemical constituents of Peucedanum ostruthium (L.) W.D.J.Koch (Apiaceae)

Abstract

Peucedanum ostruthium (L.) W.D.J.Koch (Apiaceae) is an alpine medicinal plant traditionally used as a panacea to treat various ailments. For the first time, its phytotoxic and nematotoxic properties were investigated. The inhibitory activity toward germination and seedling growth of the weeds Echinochloa oryzoides (Ard.) Fritsch and Lolium multiflorum Lam. was evaluated by two in vitro assays, carried out on filter paper and soil, using different aqueous extract concentrations (1, 10, and 20 %) and 0.25 g of powder of P. ostruthium leaves, inflorescences, and rhizomes. The study showed that all samples were more effective on L. multiflorum than E. oryzoides with p-values = 0.000 on both substrate types. Nevertheless, in all cases, the soil mitigated the P. ostruthium effects. Regarding nematicidal activity, the leaf extract was the most active against larvae and adults of the nematode Panagrolaimus rigidus. According to the motility test, their death was 85.6 ± 2.7 % and 90.5 ± 3.1 % 24 h after treatment. Lastly, NMR and UPLC-HR-MS analyses led to the identification of several compounds in the aqueous extracts, including mono- and di-substituted chlorogenic acids, flavonol glycosides, coumarins, and furanocoumarin glycosides. 5-Caffeoylquinic acid was the most abundant phenolic component in all plant organs.