Abstract
Elucidation of the phytotoxic mechanisms of thorium (Th) is important for controlling Th accumulation in crops and improving the efficiency of phytoremediation. Here, we analyzed the subcellular distribution of Th in Vicia faba seedlings and the toxic reaction of seedlings to Th (5–40 μmol·L−1) at the subcellular and cellular levels. Increasing the phosphate level in the culture medium from 0.01 to 0.1 mmol·L−1 decreased the Th accumulation by the roots by 47–57%. Th was mainly distributed in the root cell walls (94–96%) and existed mainly in the form of residue (92–94%). Th accumulation in the root was similar to the changes observed for P, Ni, Cu, and Fe. High concentrations of Th (40 μmol·L−1) induced abnormal root growth and leaf photosynthetic metabolism. At the cellular level, Th (40 μmol·L−1) induced root edge cell death and inhibited root respiration and cell mitosis. SOD, POD and CAT activities were involved in the regulation of reactive oxygen species accumulation in the roots. Untargeted metabolomics identified 580 and 262 differentially expressed metabolites in roots and leaves. At the metabolic level, its toxicological mechanism involved a severe inhibition of the expression of nucleotides in roots and leaves.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.