Abstract
This study elaborates the consequences of oxidative stress caused by copper oxide (CuO) and titanium dioxide (TiO2) nanoparticles (NPs) in Brassica juncea. Effect of these two NPs on plant physiology, reactive oxygen scavenging enzyme system (ascorbate peroxidase, catalase, superoxide dismutase), proline content and lipid peroxidation has been estimated in leaves as well as root tissues. Bioaccumulation of NPs has also been evaluated in the current study and the interrelated cascade of the enzymatic system with H2O2 production was identified. The uptake of NPs in plant leaves was confirmed by scanning electron microscopy, X-ray diffraction, and Fourier Transform Infrared Spectroscopy. Plant growth was found to be diminished with elevated levels of CuO NPs whereas TiO2 NPs had shown an opposite effect. The plant species accumulated lower concentration of NPs and displayed considerable tolerance against stress, probably due to well-organized and coordinated defense system at the root and shoot level by the intonation of antioxidative enzymes.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0550-3) contains supplementary material, which is available to authorized users.
Highlights
Nanoparticles have received a tremendous attention for their optimistic impact in many sectors of research and development
Effect of these two NPs on plant physiology, reactive oxygen scavenging enzyme system, proline content and lipid peroxidation has been estimated in leaves as well as root tissues
Retardation in growth and other harmful effects on plants could be correlated with the generation of reactive oxygen species (ROS) in the plant system which results into oxidative stress
Summary
Nanoparticles have received a tremendous attention for their optimistic impact in many sectors of research and development. TiO2 NPs are used in medicinal formulations due to germicidal and antimicrobial properties It is well-known for making corrosion-proof surfaces of metals (Wold 1993; Ellsworth et al 2000). To the best of our knowledge, so far no study has been done to investigate the role of antioxidant enzymes against NPs. This study is a dedicated attempt in this direction to investigate the oxidative stress in Brassica juncea induced by two different metal oxide nanoparticles. This study is a dedicated attempt in this direction to investigate the oxidative stress in Brassica juncea induced by two different metal oxide nanoparticles For this purpose a hydroponic system has been used to examine the accumulation and uptake of NPs by B. juncea. Evaluation of the toxic effect of these nanomaterials will help to ensure the safety of their wide range of applications, but will be useful in designing a functional materials with minimal adverse effects to plants and ecosystem
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