Phytotoxic effects of polyethylene microplastics combined with cadmium on the photosynthetic performance of maize (Zea mays L.)

Abstract

Microplastics (MPs) and cadmium (Cd) has attracted increasing attention due to their combined toxicity to terrestrial vegetation. Photosynthesis which utilizes light energy to synthesize organic substances is crucial for crop production. However, the plant photosynthetic response to the joint toxicity of MPs and Cd is still unknown. Here, we studied the effects of polyethylene (PE) MPs on the photosynthetic performance of two maize cultivars Xianyu 335 (XY) and Zhengdan 958 (ZD) grown in a Cd contaminated soil. Results showed that the leaf Cd concentration in XY and ZD reached 26.1 and 31.9 μg g−1, respectively. PE-MPs did not influence the leaf Cd content, but posed direct and negative effects on photosynthesis by increasing the malondialdehyde content, reducing the chlorophyll content, inhibiting photosynthetic capacity, disrupting the PSII donor side, blocking electron transfer in different photosystems, and suppressing the oxidation and reduction states of PSI. Transcriptomic analysis revealed that the inhibitory effect of combined PE-MPs and Cd on maize photosynthesis was attributed to suppressed expression of the genes encoding PSII, PSI, F-type ATPase, cytochrome b6/f complex, and electron transport between PSII and PSI. Using WGCNA, we identified a MEturquoise module highly correlated with photosynthetic traits. Hub genes bridging carbohydrate metabolism, amino acid metabolism, lipid metabolism, and translation provided the molecular mechanisms of PE-MPs and Cd tolerance in maize plants. The comprehensive information on the phytotoxicity mechanisms of Cd stress in the presence or absence of PE-MPs on the photosynthesis of maize is helpful for cloning Cd and PE-MP resistance genes in the future.