Abstract
Aqueous extracts of Bunias orientalis leaves showed considerable germination inhibition in petri-dish bioassays. As major B. orientalis leaf constituents with known allelopathic activity bound forms of p-coumaric, ferulic and sinapic acids as well as two C 13-norisoprenoids, i.e. 3-hydroxy-β-ionone and 3-hydroxy-5,6-epoxy-β-ionone, were identified in XAD-2 isolates. After separation of the glycosidic isolate by counter-current chromatography/HPLC, 3-hydroxy-5,6-epoxy-β-ionyl-β- d-glucoside and the glucose ester of sinapic acid were isolated in pure form and their structures elucidated from 1H and 13C NMR spectral data. The corresponding aglycones were found to be major constituents in leaf leachate solutions of B. orientalis, together with the known allelochemic loliolide.
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