PHYTOTOXIC ACTIVITY REDUCTION ASSESSMENT OF COPPER IONS THROUGH THE ONION APICAL MERISTEM

Abstract

This paper investigates the protective properties of the aqueous extract of silkworm pupae (CABG) in terms of the toxic effect of copper ions on morphometrically parameters in Allium-test. Methods: It is established that the joint use of aqueous extract of silkworm pupae and copper sulphate at a concentration of 100 µm growth inhibition of roots is not happening and even stimulated by the application of aqueous extract of silkworm pupae at a concentration of 1 ml/10 ml. Stimulation of root growth is also observed with the combined use of 1000 µm copper sulfate and aqueous extract of silkworm pupae in a concentration of 10 ml/100 ml of the solution with respect to the use of copper sulfate only. A significant decrease in the parameter ‘length of sprouts' in 1.3-6.0 times with respect to the control was observed when using copper ions in concentrations of 100-1000 microns, while at a metal concentration of 10 microns no negative effect on the development of sprouts was established. The emergence of lateral roots in 20-50% of cases observed only in the use of aqueous extract of silkworm pupae. Thus, in the study of morphometric parameters of seedlings of onion (the relative growth of roots, length of shoots, their weight) set the modifier of the protective effect of aqueous extract of silkworm pupae when used together with copper sulfate in concentrations of 10 and 100 µm. At a concentration of 10 ml of aqueous extract of silkworm pupae/100 ml solution protective function in relation to ions of copper are more severe.