Abstract
The fish disease significantly affects the aquaculture industry and causes economic harm. Antibiotics, chemotherapeutics and other synthetic pharmaceuticals are continuously used by farmers to mitigate infectious diseases. Phytotherapy without side effects is an eco-friendly, socio-economic and modern approach to mitigate disease. Toxicological and pharmacological studies are prerequisites for phytotherapy-related research and help to decide a safe dose for the main experiment and commercial aqua products. In toxicity studies, LD50 and LC50 are reliable and widely used acute toxicity parameters.
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