PHYTO-SYNTHESIS OF GOLD NANOPARTICLES USING Trewia nudiflora FRUIT EXTRACT WITH HAuCl4, CHARACTERIZATION, EVALUATION, AND ANTIMICROBIAL ACTIVITIES

Abstract

Due to their excellent catalytic, good biocompatibility, large surface area, conductivity, drug delivery agent, and antimicrobial activity properties, nanoparticles with medicinal plants are great for biomedical applications. The study was conducted from January 2017 to October 2018 at the Department of Applied Nutrition and Food Technology, Islamic University, Kushtia. The main aim of this study was to synthesize, characterize, and evaluate the antimicrobial activity of gold nanoparticles using the fruit of Trewia nudiflora as a primary source. Phyto-constituents of Trewia nudiflora were extracted with water to synthesize gold nanoparticles (AuNPs). There was a ratio of chloroauric acid (HAuCl4) solution and Trewia nudiflora fruit extract (20:1) used to optimize gold nanoparticles (AuNPs), respectively. Synthesized gold nanoparticles were confirmed by a visual color change from colorless to ruby-red, Laser beam light was passing through the synthesized gold nanoparticles to observe the Tyndall effect. UV-Vis spectroscopy was used to observe the absorption peaks between 500 and 550 nm, which is the characteristic peak range for gold nanoparticles (AuNPs). The surface morphology of the AuNPs was examined by scanning electron microscopy (SEM) and the elemental composition of the sample (AuNPs) by energy-dispersive X-ray analysis (EDAX). The antimicrobial activity of gold nanoparticles was observed in an 8.5-mm zone of inhibition against gram negative (Klebsiella sp.) and 8-mm zone of inhibition against gram positive (Micrococcus sp.) bacteria.