Phytosiderophore release by Sorghum, wheat, and corn under zinc deficiency1

Abstract

Zinc (Zn) deficiency is more common in corn (Zea mays L.) than in sorghum [Sorghum bicolor (L.) Moench] or wheat (Triticum sp.). The ability of wheat to withstand low soil Zn conditions is related to increased release of phytosiderophore from its roots. The reasons for sorghum's ability and corn's inability to utilize low levels of soil Zn have not been explored adequately. The objectives of this research were to 1) ascertain if Zn deficiency could be induced in sorghum, wheat, and corn grown in a chelator‐buffered nutrient solution and 2) determine relative releases of phytosiderophore from roots of sorghum, wheat, and/or corn under Zn‐deficiency conditions. Sorghum, wheat, and corn were grown hydroponically in the greenhouse with a chelator‐buffered nutrient solution designed to induce Zn deficiency, while supplying adequate amounts of other nutrients. Root exudates were collected over time to measure phytosiderophore release. Shoot Zn concentrations and shoot and root dry matter yields were determined also. The technique was effective for inducing Zn deficiency in sorghum, wheat, and corn, as evidenced by reduced shoot and root dry matter yields, shortened internodes, reduced shoot Zn concentrations, and plant Zn concentrations below the suggested critical values for these species. Sorghum and wheat plants increased the release of phytosiderophore in response to Zn deficiency, but com did not. The total amount of phytosiderophore released by the roots was in the order wheat>sorghum>corn. The absence of a “phytosiderophore”; response to Zn deficiency of corn, coupled with the evidence that this species requires, or at least accumulates, more Zn than wheat or sorghum, provides an explanation as to why Zn deficiencies are more prevalent for corn than wheat or sorghum under field conditions.