PHYTOREMEDIATION OF URANIUM TAILINGS: ROLE OF CHELATORS IN TRIGGERING URANIUM ACCUMULATION IN WHEAT

Abstract

Chelator-assisted phytoextraction has been proposed as a potential tool for phytoremediation of uranium contaminated tailings. The purpose of the present investigation was to test the efficiency of the four various chelators namely, citric acid (CA), oxalic acid (OA), NTA and EDTA and to screen out the most effective chelator with promising concentration of it in increasing the U uptake and accumulation for phytoremedial programmes. Three kilograms of mixture (25:75; tailing: garden soil) was filled in the earthen pots. Treatment pots were prepared by applying- 0.1, 0.5, 2.5 and 12.5 mmol kg-1 concentrations of each of the chelator (CA, OA, NTA and EDTA). Optimum concentrations of the chelators were recorded considering biomass production, tolerance index and U uptake. Each chelator produced severe toxicity symptoms at 12.5 mmol kg-1 treatment level. Lowest depression in respect of growth was observed with NTA while OA and CA were proved less toxic than EDTA. Highest inhibition was recorded in EDTA treatments at respective levels. U uptake and accumulation was concentration dependent for each of the chelator amendment. Maximum U uptake (3.4-fold) in the roots occurred at 2.5 mmol kg-1 of CA while NTA proved to be the weakest for the same purpose. Not with standing, EDTA and NTA are stronger complexion agents than CA but in contrary, the use of CA proved beneficial in U tailing phytoremediation in the present investigation. The growth of the wheat plants was affected by each of the chelator, which in general follows the order: NTA ? OA ? CA ? EDTA, whereas the order for U accumulation was recorded as- CA > EDTA > OA > NTA. On the basis of this study it can be suggested that the use CA over EDTA is better, as it is easily biodegradable, less toxic and has lower leaching risk..