Abstract
Application of phytoremediation in disinfection was studied using extracts of Tulsi, Neem and Amla in underground water in rural area of eastern Pune metropolitan region. The perception study involved understanding the potential of hydroalcoholic extracts of leaves of Tulsi, Neem and Amla in disinfection of underground water. Secondly optimizing the dose and time required for complete disinfection by the extracts. Thirdly a liquid herbal preparation for disinfection using extracts was formulated and studied, and also further used for achieving complete disinfection of onsite householdsâ water. The disinfection effect of crude powder of leaves of plants was also noted. Study area being agrarian area, a survey was taken to create awareness and highlight the outcome of the studies done so far. The 3M petri film method was used for disinfection study. The extracts of Tulsi, Neem and Amla completely disinfected 10ml of water at a dose of 10.56mg, 21.4mg and 12.03mg respectively. Herbal preparation containing 90mg extracts completely disinfected 500ml of underground water of study sites and households. The responses of crude powder were very less potent as compared to extracts. Finally, the results conclude that phytoremediation by commonly and abundantly available plants can be used for disinfection.
Highlights
We have monitored the quality of ground water by physicochemical and biological parameters at different seasonal time points in the rural area of eastern metropolitan region of Pune (Study area). 7 sites (B, C, D, F, G, H, I) of ground water near the bank of Mula-Mutha River were selected for sampling
For perception study a liquid herbal preparation was formulated using the hydroalcoholic extracts of Tulsi, Neem, and Amla in 1:1:1 ratio
This herbal preparation was studied in dose response manner by petri film method to identify the dose that can be used for perception study
Summary
We have monitored the quality of ground water by physicochemical and biological parameters at different seasonal time points in the rural area of eastern metropolitan region of Pune (Study area). 7 sites (B, C, D, F, G, H, I) of ground water near the bank of Mula-Mutha River were selected for sampling. 7 sites (B, C, D, F, G, H, I) of ground water near the bank of Mula-Mutha River were selected for sampling. In the antimicrobial study done earlier, Amla, Neem and Tulsi showed potential to disinfect the onsite underground water samples. Tulsi and Neem are the commonly available plants in the study area. The study mainly focused on validating the disinfection process by plant leaves hydroalcoholic extracts. Formulating a liquid herbal preparation and testing and using it to disinfect underground and household water in the study area. The households selected were those that were using underground water from constantly contaminated sites. A survey was conducted to understand people’s view about underground water quality highlighted by our earlier studies in their locality [1] and the use of medicinal plants for disinfection
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