Phytohormone gibberellins treatment enhances multiple antibiotics removal efficiency of different bacteria-microalgae-fungi symbionts

Abstract

To develop and characterize novel antibiotics removal biomaterial technology, we constructed three different bacteria-microalgae-fungi consortiums containing Chlorella vulgaris (C. vulgaris), endophytic bacterium, Clonostachys rosea (C. rosea), Ganoderma lucidum, and Pleurotus pulmonarius. The results showed that under treatment with 50 mg/L of gibberellins (GAs), the three bacteria-microalgae-fungi symbionts had maximal growth rates (0.317 ± 0.030 d-1) and the highest removal efficiency for seven different antibiotics. Among them, C. vulgaris-endophytic bacterium-C. rosea symbiont had the best performance, with antibiotics removal efficiencies of 96.0 ± 1.4 %, 91.1 ± 7.9 %, 48.7 ± 5.1 %, 34.6 ± 2.9 %, 61.0 ± 5.5 %, 63.7 ± 5.6 %, and 54.3 ± 4.9 % for tetracycline hydrochloride, oxytetracycline hydrochloride, ciprofloxacin, norfloxacin, sulfadiazine, sulfamethazine, and sulfamethoxazole, respectively. Overall, the present study demonstrates that 50 mg/L GAs enhances biomass production and antibiotics removal efficiency of bacteria-microalgae-fungi symbionts, providing a framework for future antibiotics-containing wastewater treatment using three-phase symbionts.