Abstract
Citrus greening or huanglongbing (HLB) is commonly known as yellow dragon disease and affects citrus production worldwide. Therefore, it has a significant impact on and deleterious effects in the agro-industrial sector. Significant efforts have been made to combat this disease and mitigate its destructive impact on citrus production, but still, there is no effective biocompatible treatment available to control HLB disorder. This study is considered the first biocompatible approach to evaluate the potential of phytogenic selenium nanoparticles (SeNPs) to improve the health of HLB-infected ‘Kinnow’ mandarin plants. Polymerase chain reactions (PCRs) with specific primers were used to detect HLB disease in ‘Kinnow’ mandarin plants, and PCR products were sequenced to identify Candidatus Liberibacter asiaticus (CLas), and accession numbers for CLas1 and CLas2, MZ851933 and MZ851934, respectively, were obtained. SeNPs were synthesized by using Allium sativum L. clove extract as a reducing, capping, and stabilizing agent and various techniques such as UV-visible spectrophotometry, energy dispersive X-rays, scanning electron microscopy (SEM), Fourier transformed infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD) were used to confirm the biogenesis of SeNPs. Different concentrations of SeNPs (25, 50, 75, and 100 mg L−1) were exogenously applied to HLB-infected ‘Kinnow’ mandarin plants and obtained spectacular results. The obtained results from the current study proved that 75 mg L−1 of SeNPs was most effective to improve the chlorophyll, carotenoids, relative water content (RWC), membrane stability index (MSI), total soluble sugar (TSS), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), total flavonoid content (TFC), and total phenolic content (TPC) and significant decrease was observed in hydrogen peroxide (H2O2), malondialdehyde (MDA), and proline (PRO) contents of HLB-infected ‘Kinnow’ mandarin plants as compared to untreated diseased citrus plants. In conclusion, these results allow us to synthesize the SeNPs formulation as a promising management strategy to treat the HLB disease in citrus plants.
Highlights
Introduction conditions of the Creative CommonsCitrus belongs to the family Rutaceae
The results showed that selenium nanoparticles (SeNPs) at 25 mg L−1 increased the chl a, chl b, total chl, and carotenoid contents by 18.21%, 12.35%, 33.70%, and 14.58%, respectively, as compared to untreated diseased ‘Kinnow’ mandarin plants (Figure 5)
The results revealed that proline content was decreased by 26.19% when SeNPs at 25 mg L−1 were exogenously applied on HLB-infected ‘Kinnow’ mandarin plants
Summary
It has a prominent position among various fruiting crops worldwide. Nanomaterials 2022, 12, 356 worldwide because of their notable nutritional status and bioactives that have healthpromoting effects. Citrus fruits contain bioactives such as carotenoids, flavonoids, essential oils, limonoids, and synephrine, which protect from various disorders, including cancer, cardiovascular, digestive, and inflammatory diseases [2,3,4]. Citrus fruits possess an important position worldwide because of their ascorbic acid content, dietary fibers, and carbohydrates such as fructose, sucrose, and glucose contents which decrease cholesterol levels and mitigate devastating problems of the digestive system. Citrus fruits play an essential role in human health and protect from various chronic maladies [5]
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.