Phytofabrication of silver nano particles using &lt;em&gt;Ocimum sanctum &lt;/em&gt;leaf extract and their antibacterial and anticancer activity through oxidative damage

Papiya Nandy,Kunal Pal,Sukhen Das,Parimal Karmakar,Debbethi Bera

doi:10.3390/mol2net-06-06781

Abstract

Silver nanoparticles (AgNP) have found prominence in different fields such as medicine, catalysis, nanoelectronics, textile field, pollution and water treatment due to their unique attributes. Applications of AgNP are increasing rapidly in the medical purpose including drug delivery, treatment, diagnosis, medical device coating. Various chemical and physical methods are used to synthesize the AgNP conventionally. However, these processes are expensive and also have side effects. To solve these problems by modification in synthesis process for safer and more efficiency, synthesis of biogenic AgNP from plant extract, known as Green Nanotechnology, have come to play a very crucial role, In this study, we have reported the green synthesis of AgNP using Ocimum sanctum (Tulsi) leaf extract, which act as reducing agent as well as capping agent. Synthesized AgNPs were characterized and their antibacterial and anticancer activities were observed. The development of brown color by the addition of Tulsi signifies the formation of silver nanoparticles. UV-Vis absorption spectroscopy, XRD and zeta potential were applied to estimate the quantitative formation of silver nanoparticles. FTIR analysis was used to reveal that the AgNPs were stabilized by eugenols, terpenes, and other aromatic compounds present in the leaf extract. The antimicrobial and anticancer properties of AgNPs were assessed by various in vitro cellular assays. Our present study confirms that AgNP can be used as a dual therapeutic option for combating pathogenic microbial strains as well as hepatocellular cancer.

Highlights

IntroductionNanotechnology is the field for development of more consistent process for the synthesis of nanomaterials more than a range of size (with good monodispersity) and chemical composition [1]
Nanotechnology is the field for development of more consistent process for the synthesis of nanomaterials more than a range of size and chemical composition [1]
The AgNPs were thoroughly charecterised with the UV-Vis, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), DLS and field emission Scanning Electron Microscope (FESEM)

Summary

Introduction

Nanotechnology is the field for development of more consistent process for the synthesis of nanomaterials more than a range of size (with good monodispersity) and chemical composition [1]. Synthesized metallic nanoparticles are enormous due to their influential applicability in various fields such as electronics, chemistry, energy, and medicine development [3]. The silver nanoparticles have a wide range of interest as they have a larger number of apposite: nonlinear optics, spectrally selective coating for solar energy absorption, good electrical conductors, bio-labeling, antibacterial materials, intercalation materials for electrical batteries as optical receptors, chemically stable materials and catalyst in chemical reactions [5, 6] In the medical field, silver and silver nanoparticles have broad applications, especially in skin ointments and creams to avoid infection of burns and open wounds [10].

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