PHYTOFABRICATION OF NICKEL OXIDE NANOPARTICLES AND THEIR ANTIOXIDANT, ANTIBACTERIAL, AND ANTICANCER ACTIVITIES

Abstract

In this study, we employed an eco-friendly approach to produce nickel oxide nanoparticles (NiO NPs) employing an aqueous leaf extract derived from Alternanthera sessilis. The resulting NiO NPs exhibited a monodispersed nature, as verified through UV-Vis spectroscopy, which exposed a clear peak at λmax 280 nm and stabilizing agents during the synthesis of A.S-NiO NPs. The XRD examination asserted the Face Centre Cubic (FCC) crystallinity of the biogenic A.S-NiO NPs. TEM examination revealed a size of 18 nm and a spherical morphology for A.S-NiO NPs. The biogenic NiO NPs demonstrated noteworthy antioxidant, antibacterial, and anticancer activities. The DPPH scavenging activity was shown to be maximum at an amount of 100 μg/ml, at 62.72%, and an IC50 value of 86.46 μg/ml. Additionally, A.S-NiO NPs exhibited superior antibacterial efficacy P. aeruginosa, E. coli, and other bacteria showed the strongest antibacterial activity. S. pyogenas, and S. aureus, resulting in Zone of Inhibition values of 16 mm, 14.5 mm, 10.6 mm, and 6.5 mm, respectively. Furthermore, A.S-NiO NPs demonstrated potential dose-dependent anticancer efficacy against HeLa and MCF-7 cancer cell lines.