Phytochrome-mediated control of prolamellar body reorganization and plastid size in mustard cotyledons.

Abstract

Abstract— The development of plastids in the palisade parenchyma cells of the cotyledons of mustard seedlings (Sinapis alba L.) was studied by electron microscopy. In darkness the etioplasts undergo a sequence of morphogenic changes previously recognized in principle in bean and barley leaves, as summarized by Rosinski, J. and W. G. Rosen (1972) Quart. Rev. Biol.47, 160–190. From 12 to 36 h after sowing, an increase in the percentage of etioplast profiles with paracrystalline prolamellar bodies can be observed. Thereafter, the degree of organization and size of the prolamellar bodies decrease. 60 h after sowing, the etioplasts show only remnants of prolamellar bodies with irregularly spaced tubules. Continuous far‐red light, which is considered to operate via phytochrome, counteracts the decay of organization of the prolamellar body and strongly increases the size of the plastids. The effect of continuous far‐red light (onset of light 36 h after sowing) can be substituted by 12 h of far‐red light given between 36 and 48 h after sowing. It is shown with red and far‐red light pulses that the morphogenic effect of long‐term far‐red light on plastid size and appearance of the prolamellar body is exclusively due to phytochrome (Pfr). Changes by light in the amounts of protochlorophyll(ide) or chlorophyll(ide) do not affect these results. The action of Pfr on the structure of the prolamellar body is a relatively fast process, occurring within 3 h. Formation of thylakoids does not seem to be under phytochrome control. Rather, this response seems to be related to the protochlorophyll(ide)→ chlorophyll(ide) a transformation.