Abstract
Chloroplasts isolated from 5-day-old barley shoots by differential centrifugation have l-phenylalanine ammonia lyase (PAL) activity. Based on chlorophyll/PAL ratios, the chloroplasts have about 29% of the PAL of the whole shoot. When plastid preparations are fractionated by discontinuous sucrose gradient centrifugation, highest PAL activity is found at the interface containing predominantly intact plastids. Continuous sucrose gradient centrifugation shows a correlation between chlorophyll content and PAL levels. PAL activity in 5-day-old etioplasts is increased ca 40% by giving the plants 4 min of red light 5 hr before harvest. Far-red light reverses the red light effect and demonstrates phytochrome control of barley plastid PAL.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
