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Abstract
Introduction: In this study, Falcataria moluccana as a plant of West Java community forest was evaluated for its phytochemical content, characterization of secondary metabolites through GC/MS analysis, and antioxidant activity with the DPPH method. Methods: The extraction of F. moluccana twig used maceration with methanol solvent. Phytochemical compounds in F. moluccana methanolic extract were identified using Gas Chromatography-Mass Spectrometry (GC/MS). The antioxidant activity was tested against 2,2-diphenyl1- picrylhydrazyl (DPPH). Results: The phytochemical screening of F. moluccana methanolic extract showed the presence of phenolics, flavonoids, steroids, terpenoids, saponins, and tannins. The results of GC/MS analysis showed that the highest abundance was α-terpinolenic from the terpenoid group with a retention time of 6.776 minutes and a percentage area of 25.85%. Total phenolic content in methanolic extract of F. moluccana was 145.21 mg GAE/g, total flavonoid was 95.39 mg QE/g while antioxidant activity (IC50) was 12.60 ppm. Conclusion: F. moluccana has potential as natural antioxidant and its active compounds can be developed as pharmaceutical raw materials.
Highlights
MATERIAL AND METHODSPlants are very rich in secondary metabolite components with specific bioactivity for human needs in the health sector
In this study, Falcataria moluccana as a plant of West Java community forest was evaluated for its phytochemical content, characterization of secondary metabolites through Gas Chromatography-Mass Spectrometry (GC/MS) analysis, and antioxidant activity with the diphenyl1- picrylhydrazyl (DPPH) method
The phytochemical screening of F. moluccana methanolic extract showed the presence of phenolics, flavonoids, steroids, terpenoids, saponins, and tannins
Summary
Plants are very rich in secondary metabolite components with specific bioactivity for human needs in the health sector. One of the potential plants as a source of natural antioxidant compounds is F. moluccana which is a multipurpose species in Southeast Asian plantations, especially Indonesia and Malaysia.[8] The purpose of this study was to find out phytochemical content, characterization of secondary metabolites by GC/MS analysis, and antioxidant activity of F. moluccana methanolic extract. The extract solution was produced at a concentration of 500 mg/L in the form of the extract dissolved in methanol, 5 mL extract was added with 0.3 mL 5% NaNO2. 1 mL of F. moluccana methanolic extract with 100, 200, 300, 400, and 500 ppm concentrations were mixed with 2 mL of 0.002% DPPH solution and rested for 30 minutes. IC50 (inhibitory concentration) was determined by calculating the concentration with 50% inhibition from linear regression equation
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