Abstract
In the present research work the phytochemical and Chromatographic separation of bio active compounds Berberis lycium occurred. Phytochemical investigation of the CH2 Cl2 /CH3 OH (1:1) extract ethanol of root shown the presence of terpenoids, flavonoids, saponins, anthraquinones and alkaloids, steroids and tannins was absent. Chromatographic separation of CH2 Cl2 /CH3 OH (1:1) root ethanol extract yielded methyl 9, 10-dihydro-3, 8-hydroxy-6-methyl-9, 10-dioxoanthracene-2- carboxylate, isolated for the first time from the species of Berberis lycium. With the help of spectroscopic methods (IR, 1 H NMR, 13C NMR, and DEPT-135 and UV-Vis,) Partial characterization of the isolated compound was done.
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