Abstract

The raw material base of medicinal plants in Ukraine is sufficient only for half of the pharmacopoeial species. Most part of the medicinal plants is growing in insufficient quantities and there is a need for their import. In conditions of import dependence and a shortage of domestic plant materials, the search for new sources of biologically active substances among the representatives of the flora of Ukraine is an urgent task of modern pharmaceutical science.The aim of the work – conduct a comparative phytochemical study of the leaves of S. grandiflora and S. officinalis to establish the possibility of using the non-pharmacopeia species in pharmaceutical and medical practice.Materials and methods. The objects of the study are the leaves of S. grandiflora and S. officinalis, which was harvested in the botanical garden of the Lviv National University named after I. Franko. The study of macro- and microelement composition in the leaves of S. officinalis and S. grandiflora was carried out by atomic emission spectrographic method. Determination of the qualitative composition and quantitative content of the main groups of biologically active substances was carried out by HPLC. Quantitative determination of phenolic compounds was also carried out by spectrophotometric method.Results. The content of 15 micro and macro elements was found in both studied species. In the leaves of S. officinalis and S. grandiflora, 15 amino acids and 8 saponins were identified. Using HPLC, the qualitative composition and quantitative content of phenolic substances in the leaves of S. officinalis and S. grandiflora (13 and 9 compounds, respectively) was established.Discussion. The dominant macro and micro elements in the studied species were silicon, phosphorus, magnesium, calcium, sodium, and potassium. The total content of trace elements in the leaves of S. grandiflora is 1.67 times greater than in the pharmacopeia plant S. officinalis. The dominant amino acids in the leaves of both species are glutamic acid, aspartic acid, valine and leucine.The dominant saponins in the leaf of S. officinalis were ursolic and oleanolic acids, the total content of which is 75.82 %. In the leaves of S.grandiflora, ursolic and euskapic acids were dominant, with a total content of 63.25 %.The total flavonoid content is higher in S. officinalis leaf and is 4.90 mg / g. The total content of hydroxycinnamic acids is highest in the leaf of S. grandiflora and is 4.49 mg / g, which is 221.18 % (2.21 times) higher than in the pharmacopeia plant S. officinalis (2.03 mg / g). The total highest content of caffeic acid derivatives prevails in the Salvia officinalis leaf (0.77 mg / g). The highest content of the sum of all detected phenolic compounds is specified for S. officinalis leaves and amounts to 6.93 mg / g.Conclusions. As a result of a comparative phytochemical study of the leaves of S. grandiflora and S. officinalis, it was established that S. grandiflora is a promising species for introduction into medical and pharmaceutical practice, namely, as a source of phenolic compounds

Highlights

  • The pharmaceutical market for synthetic drugs is showing rapid growth

  • The total content of flavonoids is highest in leaves of S. officinalis and is 4.90 mg / g

  • Comparative pharmacognostic and pharmacological studies of S. grandiflora and S. officinalis leaves revealed that S. grandiflora is a promising species for introduction into medical and pharmaceutical practice as a source of phenolic compounds, in particular hydroxycinnamic acids

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Summary

Introduction

The pharmaceutical market for synthetic drugs is showing rapid growth. At the same time, there remains high level of topicality of herbal medicines research. New drugs based on biologically active substances of plant origin are emerging every year [1, 2]. The market of medicinal herbal raw materials in Ukraine is rapidly developing, as evidenced by the development and increase in the number of enterprises of all units from the cultivation and harvesting of medicinal products to the production of finished products. In today's conditions, the market of medicinal plants of Ukraine is dominated by imported raw materials, which exacerbates the issue of import substitution to ensure the medical safety of the country [3, 4]. In conditions of import dependence and scarcity of domestic medicinal plant raw materials, the search for new sources of biologically active substances among the representatives of the flora of Ukraine is an urgent task of modern pharmaceutical science [1, 5]

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