Phytochemical studies on cyanidin 3-O glucoside•HCl from the ripened fruit pulp of Terminalia catappa

Abstract

Context :Terminalia catappa (Combretaceae) is a large tropical tree grows mainly in the tropical regions of Asia, Africa, and Australia. The fruits a drupe 5-7 cm long and 3-5.5 cm broad, green at first, then yellow, and finally red when ripe, containing a single seed. Ripened fruit pulp contains anthocyanins. Objectives: To extract and isolate active anthocyanin from fruit pulp of Terminalia catappa. And characterize isolated cyanidin-3-0-glucoside by spectral analysis.Methods: An easy and efficient technique for isolating and extracting anthocyanin from Terminalia catappa ripen fruit pulp. Besides the total phenolic, flavonoid, anthocyanin, and reducing sugar content of the extract, the antioxidant capacity of T.catappa fruit pulp was evaluated and by DPPH, H2O2 methods. Characterization of cyanidin-3-o-glucoside done by UV, IR spectral analysis. While single crystal structure and elemental detection done by EDS analysis. And further structural confirmation done by LCMS analysis.Result: Fresh extract contain 26.17 % and isolated compound contain 83.79% total anthocyanin content, the total phenolic content was found to be 1.58 % in fresh extract as compared with gallic acid standard. 34.48 % total reducing sugar content and 54.50 % total flavonoid content observed.