Phytochemical Studies and Identification of Bioactive Compounds using High-performance Liquid Chromatography and Gas Chromatography- Mass Spectrometry of Leaf Extract of Jatropha curcas

Abstract

Chronic, recurring, and remitting inflammatory disease with an unknown cause is inflammatory bowel disease (IBD). It is a condition that affects people throughout their lives and has many consequences because it is clinically common. Numerous risk factors are still unknown, and the pathophysiology of IBD is still not fully understood. There are a variety of side effects associated with conventional ulcerative colitis treatment. Globally, there is a rise in the use of herbal therapy for IBD. The medicinal plant Jatropha curcas (L.) belongs to the Euphorbiaceae family. It is a biofuel plant with a few pharmacological effects that have been researched. The study’s purpose was to discover and investigate the bioactive components in an ethanolic extract of J. curcas. The leaves of L. J. curcas were gathered, shade dried, crushed up, and gradually extracted with ethanol using the soxhlet percolation method. By using fourier-transform infrared spectroscopy (FTIR) and high-performance liquid chromatography (HPLC), the phytoconstituents in the Jatropha leaf extract were confirmed. Along with the interpretation of mass spectra, the isolated chemicals were identified by contrasting their peak areas and retention times. Due to the discovery of possible, bioactive components as a result of the current inquiry, we are now able to investigate biological activity. After that, gas chromatography-mass spectrometry (GC-MS) was used to analyze the crude extracts. A large variety of secondary metabolites were detected in the extracts’ profile, which was then spectroscopically described. The presence of phytoconstituents, including flavonoids, tannins, and triterpenoids significantly reduces internal inflammation and lessens damage to the intestinal mucosa. Herbal treatments work through a variety of processes, including immunological control and antioxidant activities.