Abstract
The present study was designed to detect the class of compound by phytochemical evaluation and perform the high performance thin layer chromatography (HPTLC) fingerprint analysis of hexane extract of P. quadrifida (HEPQ), chloroform extract of P. quadrifida (CEPQ), ethyl acetate extract of P. quadrifida (EAEPQ) and ethanol extract of P. quadrifida (EEPQ). The successive extraction based on eluotropic solvent system was utilised for extraction. The preliminary phytochemical screening was carried out for all the extract. For the HPTLC fingerprint profiling the precoated Merck silica plates were used as stationary phase while, mobile phase utilised was Toluene: Chloroform: Ethanol (5:4:1 v/v/v). The scanning was done at White light, 254 nm and 366 nm. The presence of steroids, coumarins, flavonoids, glycosides, saponins, coumarins was found in all 4 extracts, except the terpenoids which were present in HEPQ and EAEPQ. The fingerprinting profile was successfully obtained for all four extracts of P. quadrifida. This profiling data can thus be utilized for quality control of the P. quadrifida both on qualitative and quantitative basis.
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