Phytochemical Standardization of Serankottai nei (a Siddha drug from milk extract of Semecarpus Anacardium nuts) and its in-vitro antitubercular activity against H37Rv strain

Abstract

International Journal of Pharmacology and Clinical Sciences,2016,5,1,17-24.DOI:10.5530/ijpcs.5.1.4Published:March 2016Type:Research ArticleAuthors:G Senthilvel, Arul Amuthan, and KN Sunil Kumar Author(s) affiliations:G Senthilvel1, Arul Amuthan2*, KN Sunil Kumar3 1Research Officer (Siddha)-Scientist-2, Central Council for Research in Siddha (CCRS)-Posted at Ministry of AYUSH, Government of India, AYUSH BHAWAN, New Delhi-110023, INDIA 2Department of Pharmacology, Melaka Manipal Medical College and Team Leader, Center for Integrative Medicine and Research, Manipal University, Karnataka-576104, INDIA 3Senior Research Officer-Pharmacognosy, S.D.M Centre for Research in Ayurveda and Allied Sciences, Udupi-574118, Karnataka, INDIA Abstract:Background: Serankottai nei is a popular Siddha drug, used in the treatment of lung infections including tuberculosis, autoimmune joint diseases (rheumatoid arthritis, degenerative osteoarthritis), cancers and neurological pain. Objective: To standardize serankottai nei and to screen its in-vitro antitubercular activity of in H37Rv strain. Materials and Methods: Serankottai nei, a medicated ghee preparation was procured from the SKM Siddha and Ayurveda Co India Ltd, Erode, Tamil Nadu. Unsaponifiable matter (USM) from the ghee preparation was separated and preliminary phytochemical screening was done. Further, USM was dissolved in 10 ml of chloroform and 3 and 6 μl of the above sample was applied for HPTLC fingerprint, which was developed in toluene: ethyl acetate (9.0:1.0). The developed plates were scanned under UV 254 nm, 366 nm, 540 nm and 620 nm post derivatization. Rf, colour of the spots and densitometric scan were recorded. Different doses of USM were screened for in-vitro antitubercular activity against H37Rv strain using Alamar Blue Dye method. Results: Phytochemical screening of 8% w/w USM obtained from serankottai nei showed presence of alkaloid, phenol, steroid and terpenoid. In HPTLC, there were 15, 5 and 7 peaks at 254 nm, 366 nm and 620 nm respectively. The Minimum Inhibitory Concentration (MIC) against H37Rv strain of pyrazinamide, streptomycin, ciprofloxacin were 3.125, 6.25 and 3.125 μg/ml respectively. Whereas, the MIC of serankottai nei was 1.6 μg/ml, which was almost 25 to 50% of standard drugs. Conclusion: Serankottai nei has shown promising antitubercular activity in in-vitro study. Thus, Semecarpus anacardium could be a suitable candidate for a new herbal based antitubercular drug. Keywords:Antitubercular, Semecarpus anacardium, Serankottai nei, Siddha Medicine, TuberculosisView:PDF (2.97 MB) PDFClick here to download the PDF file. Images Antitubercular screening of Serankottai nei by Alamar Blue assayagainst H37Rv strain