Phytochemical screening of white oyster mushroom (Pleurotus ostreatus)

Abstract

Event Abstract Back to Event Phytochemical screening of white oyster mushroom (Pleurotus ostreatus) preparations Santun B. Rahimah1, 2*, Dhiah D. Djunaedi2, Arto Y. Soeroto2 and Tatang Bisri2 1 Bandung Islamic University, Indonesia 2 Padjadjaran University, Indonesia Background Pleurotus ostreatus is a popular commercial mushroom that contains very high nutrients and bioactive compounds with therapeutic effects. The ethanolic extractions seem to be the most active preparation. This study aimed to compare the phytochemical analysis of fresh, dry and ethanolic extracts of Pleurotus ostreatus. Methods The fresh (FPM), dry (DPM) and ethanolic extracts [macerated with 70% (EE70) and 96% ethanol (EE96)] of Pleurotus ostreatus were used in the study. The phytochemical analysis was conducted using the Dragendorf and Mayer reagents for alkaloids, FeCl3 and gelatin tests for polyphenolic and tannins, the Sawkosky and Lieberman methods for steroids and triterpenoid, an ammonium and Shinoda tests for flavonoids, foam test for saponin and NaOH reagent was used for the Quinone. Results The phytochemical screening showed that flavonoid, phenolic compounds, tannin, saponin, alkaloids, and steroids were detected in the FPM, DPM, EE70 and also in the EE96. Alkaloid however, was not identified by the Mayer Reagent in the FPM and DPM. The DPM and EE70 seemed to have the highest amount of saponin based on the foam output. Meanwhile, steroids and flavonoids were detected at a higher level in the EE96, based on the strength of visible color. However, triterpenoids and quinones could not be identified. Conclusion The process of drying Pleurotus ostreatus did not reduce the content of active substances. The slight differences in the phytochemical screening obtained in all samples may be due to the influence of water content and polarity of the active substances. The polar active substances seem to be more soluble in the EE70 than the EE96. The higher the bioactive substances in the preparation, the bio-therapeutic effects become more significant. Acknowledgements Thank the Lembaga Pengelola Dana Pendidikan (LPDP) and the Bandung Islamic University’s Medical Faculty and Padjadjaran University in Bandung for helping in the research process. Keywords: Pleurotus ostreatus (jacq.) p. kumm, phytochemical, dry plants material, fresh plants material, Ethanolic extract Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Miscellaneous Citation: Rahimah SB, Djunaedi DD, Soeroto AY and Bisri T (2019). Phytochemical screening of white oyster mushroom (Pleurotus ostreatus) preparations. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00014 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 05 Nov 2018; Published Online: 17 Jan 2019. * Correspondence: Mrs. Santun B Rahimah, Bandung Islamic University, Bandung, Indonesia, santunbr94@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Santun B Rahimah Dhiah D Djunaedi Arto Y Soeroto Tatang Bisri Google Santun B Rahimah Dhiah D Djunaedi Arto Y Soeroto Tatang Bisri Google Scholar Santun B Rahimah Dhiah D Djunaedi Arto Y Soeroto Tatang Bisri PubMed Santun B Rahimah Dhiah D Djunaedi Arto Y Soeroto Tatang Bisri Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.