Abstract
BackgroundMicrobial resistance, diabetes mellitus, and obesity are global health care problems that have posed a serious threat to both human and environmental ecosystems. The goals of the present investigations are to investigate the phytoconstituents, antilipase, anti-α-amylase, and antimicrobial activity of Orobanche aegyptiaca Pers. (OA) from Palestine.MethodsIdentification of the phytoconstituents of OA plant petroleum ether, methylene chloride, chloroform, acetone, and methanol extracts were conducted using pharmacopeia’s methods, while porcine pancreatic lipase and α–amylase inhibitory activities were examined using p-nitrophenyl butyrate and 3,5-dinitro salicylic acid methods, respectively. Moreover, the antimicrobial activity was evaluated utilizing broth microdilution assay against eight bacterial and fungal strains.ResultsThe phytochemical screening results showed that the methanol extract of the OA plant is rich in phytochemical components, also this extract has powerful antilipase potential with an IC50 value of 19.49 ± 0.16 μg/ml comparing with the positive control (Orlistat) which has antilipase activity with IC50 value of 12.3 ± 0.35 μg/ml. Moreover, the methanol and chloroform extracts have powerful α-amylase inhibitory activity with IC50 values of 28.18 ± 0.22 and 28.18 ± 1.22 μg/ml, respectively comparing with Acarbose which has α-amylase inhibitory activity with IC50 dose of 26.3.18 ± 0.28 μg/ml. The antibacterial results showed that the methylene chloride extract exhibited the highest antibacterial activity among the other OA plant extracts with a MIC value of 0.78 mg/ml against S. aureus, while, the methylene chloride, petroleum ether, and chloroform extracts of the OA plant showed potential antifungal activity against C. albicans strains with MIC value of 0.78 mg/ml.ConclusionThe OA methanol and chloroform extracts could be excellent candidates as antilipase and anti-α-amylase bioactive materials. In addition, methylene chloride, petroleum ether, and chloroform extracts could be potential natural antimicrobial products.
Highlights
Phytotherapy is a famous ancient type of therapeutics that arises from plants that positively affect the human body for various diseases
Phytochemical screening The preliminary phytochemical screening tests revealed the presence of terpenoids and tannins in Orobanche aegyptiaca Pers. (OA) petroleum ether extract while reducing sugars and terpenoids were found in the methylene chloride extract
The results revealed that the methanol OA extract has powerful anti-lipase potential followed by acetone, chloroform, and petroleum ether, with IC50 values of 19.49 ± 0.16, 26.3 ± 0.23, 63.09 ± 0.2 and 199.5 ± 0.2 μg/ml, respectively, comparing with the positive control (Orlistat) which has antilipase activity with an IC50 value of 12.3 ± 0.35 μg/ml
Summary
Phytotherapy is a famous ancient type of therapeutics that arises from plants that positively affect the human body for various diseases. Several studies from different global regions recorded the increase in the use of plants to cure various human diseases [2]. In 2016, the statistics showed that more than1.9 a billion adults aged 18 years and over were overweight. It found that over 650 million of them were obese. Diabetes mellitus, and obesity are global health care problems that have posed a serious threat to both human and environmental ecosystems. The goals of the present investigations are to investigate the phytoconstituents, antilipase, anti-α-amylase, and antimicrobial activity of Orobanche aegyptiaca Pers. (OA) from Palestine
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