Phytochemical screening and Invitro Anticancer activity of Lonicera ligustrina leaf extract on Breast and Colorectal carcinoma cell lines

Abstract

Cancer can be described as a disease characterized by groups of aberrant cells that undergo uncontrolled proliferations in the absence of cell cycle regulation. The metastasize cancer cells spread to various locations in the body where their uncontrolled growth invades the normal tissue. Cancer comprises more than hundred different diseases, including malignant tumors of different sites such as breast, cervix, stomach, intestines, colon, lung, mouth etc. There are numerous factors responsible for the cause, like genetic, tobacco, chemicals, environmental factors, hormones etc. Cancer detection is described by their pathological grade and clinical stage. Thus we have made an attempt to use Lonicera ligustrina herbal extract to check the efficacy against breast and colorectal carcinoma cell lines. The present study aimed to evaluate preliminary phytochemical screening and invitro anticancer potential activities against MCF-7(breast) and HCT-116 (colorectal) cancer cell lines. Samples shown significant activity at its higher concentration and the IC50 value of breast cell lines 24.45µM (MCF-7) and colorectal cell lines 9.210µM (HCT-116) inhibition. Standard Doxorubicin tested against MCF-7 and HCT-116 cell lines showed IC50 value of 18.76µM and 16.89µM inhibition. Therefore, a dynamic view of IC50 values and the methods used to detect the density-dependent IC50 spectrum of a cancer cell line (primary or passaged) established. This view will benefit patients and the cancer research community as a whole.